Table 1 Primers used for PCR amplification, Northern-blot and selection of cDNA clones.
From: Characterization of the transcripts of human cytomegalovirus UL144
Name of Primers | Sequence | Position in UL144 ORF | Position in GQ981646 |
|---|---|---|---|
144Rev1 | 5'-ttacagggtgcggtagaaaatt-3 | 510-531 | 12093-12114 |
144Rev2 | 5'-acaaccaggctagagtatgacgacc-3' | 388-412 | 11971-11995 |
144For1 | 5'-gtgaagatggctgactatcct-3' | -182~-162 | 11401-11421 |
144For2 | 5'-atgaagcctctggtgatgct-3' | 1-20 | 11584-11603 |
144For3 | 5'-aaaatgtgtaagcccgatga-3' | 61-80 | 11644-11663 |
144Prob | 5'-taatacgactcactatagggttacagggtgcggtagaaaattttg-3' | 507-531 | 12090-12114 |