Effective inhibition of foot-and-mouth disease virus (FMDV) replication in vitro by vector-delivered microRNAs targeting the 3D gene

Table 1 Inserted sequences in miRNA-expressing plasmids

Name	Inserted sequence (5'-3')		Position in 3D gene
3D657	Top strand	TGCTGAATCTTTGCCAATCAACGTCAGTTTTGGCCACTGACTGACTGACGTTGTGGCAAAGATT	657-678
	Bottom strand	CCTGAATCTTTGCCACAACGTCAGTCAGTCAGTGGCCAAAACTGACGTTGATTGGCAAAGATT C
3D715	Top strand	TGCTGATCAAAGGCCGAATAGTCCACGTTTTGGCCACTGACTGACGTGGACTACGGCCTTTGAT	715-736
	Bottom strand	CCTGATCAAAGGCCGTAGTCCACGTCAGTCAGTGGCCAAAACGTGGACTATTCGGCCTTTGAT C
3D983	Top strand	TGCTGAGATCATGGTGTAAGTGTCCAGTTTTGGCCACTGACTGACTGGACACTCACCATGATCT	983-1004
	Bottom strand	CCTGAGATCATGGTGAGTGTCCAGTCAGTCAGTGGCCAAAACTGGACACTTACACCATGATCT C
3D1311	Top strand	TGCTGTCAAAGAGACGCCGGTACTCGGTTTTGGCCACTGACTGACCGAGTACCCGTCTCTTTGA	1311-1332
	Bottom strand	CCTGTCAAAGAGACGGGTACTCGGTCAGTCAGTGGCCAAAACCGAGTACCGGCGTCTCTTTGA C
Negative control	Top strand	TGCTGAAATGTACTGCGCGTGGAGACGTTTTGGCCACTGACTGACGTCTCCACGCAGTACATTT	NO
	Bottom strand	CCTGAAATGTACTGCGTGGAGACGTCAGTCAGTGGCCAAAACGTCTCCACGCGCAGTACATTTc

Bold and underlined letters represent sense sequences of pre-miRNAs derived from the 3D gene.

ISSN: 1743-422X