Figure 4

Decrease of virus yield in BHK-21 cells transfected with the miRNA expression plasmids. BHK-21 cells transfected with each miRNA expression plasmid were infected with FMDV O/CHA/99. Cell cultures were collected at 24 h and 48 h after infection, and the virus titer (TCID50) was determined three times on BHK-21 cells. Error bars indicate standard deviations.