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Figure 1 | Virology Journal

Figure 1

From: Effective inhibition of foot-and-mouth disease virus (FMDV) replication in vitro by vector-delivered microRNAs targeting the 3D gene

Figure 1

Schematic presentations of miRNA targeting sequences and miRNA expression cassette. (A) The FMDV genome contains a unique open reading frame. The black arrows underneath indicate the sites targeted by miRNAs. (B) Pre-miRNA oligonucleotides corresponding to each of the target sequences in 3D was inserted under the control of Pcmv and a transcriptional termination signal (TK pA). As a result, the pre-miRNA forms an intramolecular stem-loop structure similar to the structure of endogenous pre-miRNA that is processed by the endogenous Dicer enzyme into a 22 nt mature miRNA.

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Virology Journal

ISSN: 1743-422X