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Figure 2 | Virology Journal

Figure 2

From: A reporter system for assaying influenza virus RNP functionality based on secreted Gaussia luciferase activity

Figure 2

Heat-sensitivity of Gluc and Fluc. (A) RNP activity in polI-Gluc- and pYH-Fluc-transfected 293T cells after heat-treatment. 293T cells were transfected with plasmids encoding the PB2, PB1, PA, and NP segments of influenza G1 (H9N2) plus reporter plasmids polI-Gluc and pYH-Fluc. Supernatants and corresponding cell lysates were heated at 65°C for 10 or 30 min, and then Gluc and Fluc activities were assayed. (B) RNP activity in G1-infected A549 cells after heat treatment. Polymerase activity assays were performed in A549 cells transfected with vRNA Gaussia and firefly reporter plasmids and then infected with H9N2 at 6 h post-transfection. Supernatants and corresponding cell lysates were harvested for assay of Gluc and Fluc activity at appropriate times. Data are expressed as standard errors of the mean (SEM) from three independent experiments performed in triplicate.

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Virology Journal

ISSN: 1743-422X