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Table 1 Dengue virus deletion mutants in the first TMD of the E protein of DV2

From: Structural mutants of dengue virus 2 transmembrane domains exhibit host-range phenotype

SEQUENCE E-T1 Domain

Foci

Critical AA Vero

Critical AA C6/36

   

Vero

C6/36

  
 

WT DV2

452SWTMKILIGVIITWIG467

++

++

  

1

ΔIG466-67

SWTMKILIGVIITW--

-

-

 

2aa

2

ΔW465

SWTMKILIGVIIT-IG

+

-

  

3

ΔT464

SWTMKILIGVII-WIG

+

-

 

T 464

4

T464A

SWTMKILIGVIIAWIG

+

+

  

5

ΔIT463-64

SWTMKILIGVI--WIG

+

-

 

2aa

6

ΔIIT462-64

SWTMKILIGV---WIG

-

-

 

T 464

7

ΔVIIT461-64

SWTMKILIG----WIG

+

-

 

T 464

8

ΔGVIIT461-64

SWTMKILI-----WIG

+

-

5aa

5aa

9

ΔI463

SWTMKILIGVI-TWIG

+

-

 

I 463

10

ΔII462-63

SWTMKILIGV--TWIG

+

-

 

2aa

11

ΔVII461-63

SWTMKILIG---TWIG

-

-

  

12

ΔGVII460-63

SWTMKILI----TWIG

+

+

  

13

ΔIGVI459-64

SWTMKIL----ITWIG

+

-

  

14

ΔIGVII459-63

SWTMKIL-----TWIG

+

-

5aa

5aa

15

ΔV461

SWTMKILIG-IITWIG

+

+

  

16

ΔGV460-61

SWTMKILI--IITWIG

+

-

 

2aa

17

ΔIGV459-61

SWTMKIL---IITWIG

+

+

  

18

ΔLIGV458-61

SWTMKI----IITWIG

+

-

  

19

ΔILIGV457-61

SWTMK-----IITWIG

-

-

5aa

5aa

20

ΔG460

SWTMKILI-VIITWIG

+

+

  

21

ΔIG459-60

SWTMKIL--VIITWIG

+

-

 

2aa

22

ΔLIG458-60

SWTMKI---VIITWIG

+

+

  

23

ΔILIG457-60

SWTMK----VIITWIG

+

+

  

24

ΔILI457-59

SWTMK---GVIITWIG

+

+

  

25

ΔIL457-58

SWTMK--IGVIITWIG

-

-

 

2aa

26

ΔI457

SWTMK-LIGVIITWIG

+

-

 

I 457

27

ΔKI456

SWTM--LIGVIITWIG

+

-

 

2aa

28

ΔK456

SWTM-ILIGVIITWIG

-

-

K 456

K 456

29

K456A

SWTMAILIGVIITWIG

+

+

  

30

ΔMK455-56

SWT--ILIGVIITWIG

+

-

 

2aa

31

ΔM455

SWT-KILIGVIITWIG

-

-

M 455

M 455

32

ΔTM454-55

SW--KILIGVIITWIG

+

-

 

2aa

33

ΔT454

SW-MKILIGVIITWIG

+

+

  

34

ΔW453

S-TMKILIGVIITWIG

+

+

  
  1. Deletions in the E-T1 domain of DV2 were produced by site-directed PCR-based mutagenesis. The mutant viruses were screened by a DV2 mutant transfection by focus assay performed on 24-well plates in both mammal (Vero) and insect (A. Albopictus, C6/36) cells. Foci were visualized immuno-histochemically. Mutant viruses able to produce foci of infection were scored as positive (+) or negative (-) after 10 days of infection. Virus mutants giving foci in both hosts were amplified and titered to determine phenotype. AA; amino acid.