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Table 1 Dengue virus deletion mutants in the first TMD of the E protein of DV2

From: Structural mutants of dengue virus 2 transmembrane domains exhibit host-range phenotype

SEQUENCE E-T1 Domain Foci Critical AA Vero Critical AA C6/36
    Vero C6/36   
  WT DV2 452SWTMKILIGVIITWIG467 ++ ++   
1 ΔIG466-67 SWTMKILIGVIITW-- - -   2aa
2 ΔW465 SWTMKILIGVIIT-IG + -   
3 ΔT464 SWTMKILIGVII-WIG + -   T 464
4 T464A SWTMKILIGVIIAWIG + +   
5 ΔIT463-64 SWTMKILIGVI--WIG + -   2aa
6 ΔIIT462-64 SWTMKILIGV---WIG - -   T 464
7 ΔVIIT461-64 SWTMKILIG----WIG + -   T 464
8 ΔGVIIT461-64 SWTMKILI-----WIG + - 5aa 5aa
9 ΔI463 SWTMKILIGVI-TWIG + -   I 463
10 ΔII462-63 SWTMKILIGV--TWIG + -   2aa
11 ΔVII461-63 SWTMKILIG---TWIG - -   
12 ΔGVII460-63 SWTMKILI----TWIG + +   
13 ΔIGVI459-64 SWTMKIL----ITWIG + -   
14 ΔIGVII459-63 SWTMKIL-----TWIG + - 5aa 5aa
15 ΔV461 SWTMKILIG-IITWIG + +   
16 ΔGV460-61 SWTMKILI--IITWIG + -   2aa
17 ΔIGV459-61 SWTMKIL---IITWIG + +   
18 ΔLIGV458-61 SWTMKI----IITWIG + -   
19 ΔILIGV457-61 SWTMK-----IITWIG - - 5aa 5aa
20 ΔG460 SWTMKILI-VIITWIG + +   
21 ΔIG459-60 SWTMKIL--VIITWIG + -   2aa
22 ΔLIG458-60 SWTMKI---VIITWIG + +   
23 ΔILIG457-60 SWTMK----VIITWIG + +   
24 ΔILI457-59 SWTMK---GVIITWIG + +   
25 ΔIL457-58 SWTMK--IGVIITWIG - -   2aa
26 ΔI457 SWTMK-LIGVIITWIG + -   I 457
27 ΔKI456 SWTM--LIGVIITWIG + -   2aa
28 ΔK456 SWTM-ILIGVIITWIG - - K 456 K 456
29 K456A SWTMAILIGVIITWIG + +   
30 ΔMK455-56 SWT--ILIGVIITWIG + -   2aa
31 ΔM455 SWT-KILIGVIITWIG - - M 455 M 455
32 ΔTM454-55 SW--KILIGVIITWIG + -   2aa
33 ΔT454 SW-MKILIGVIITWIG + +   
34 ΔW453 S-TMKILIGVIITWIG + +   
  1. Deletions in the E-T1 domain of DV2 were produced by site-directed PCR-based mutagenesis. The mutant viruses were screened by a DV2 mutant transfection by focus assay performed on 24-well plates in both mammal (Vero) and insect (A. Albopictus, C6/36) cells. Foci were visualized immuno-histochemically. Mutant viruses able to produce foci of infection were scored as positive (+) or negative (-) after 10 days of infection. Virus mutants giving foci in both hosts were amplified and titered to determine phenotype. AA; amino acid.