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Figure 2 | Virology Journal

Figure 2

From: A baculovirus dual expression system-based vaccine confers complete protection against lethal challenge with H9N2 avian influenza virus in mice

Figure 2

Characterization of BV-Dual-HA. PK-15 cells were transduced with BV-Dual-HA (A) or AcMNPV-WT (B) at an MOI of 10. At 48 h post-transduction, cells were fixed with absolute methanol, and processed for indirect immunofluorescent assay. Bound antibodies were detected by FITC-labeled anti-mouse IgG by fluorescence microscopy (green). Original magnification × 200. Electron micrograph of recombinant baculovirus displaying HA on the viral envelope. BV-Dual-HA (C) and AcMNPV-WT (D) were treated with anti-HA monoclonal antibodies, followed by labeling with anti-mouse IgG-gold conjugate. One end of the viral envelopes was strongly labeled with gold particles (arrows). Bars - 100 nm.

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Virology Journal

ISSN: 1743-422X