Establishment of real-time quantitative reverse transcription polymerase chain reaction assay for transcriptional analysis of duck enteritis virus UL55 gene

Table 1 Sequences of primers

Name	Type	Sequences (5' to 3')	Length(nt)	Amplicon (bp)
P1	Forward	GGATCC ATGGCCGACGCGAAGGCGGT	26	799
P2	Reverse	CTCGAG GCTTGGGTCTTTACTTTTTGCGCGGAAC	34
P3	Forward	TATTCTTCTGCGGGCTCA	18	124
P4	Reverse	CATAGACGATGCTCC	15
P5	Forward	CCGGGCATCGCTGACA	16	178
P6	Reverse	GGATTCATCATACTCCTGCTTGCT	24

The underlined sequences of P1 and P2 are BamH I and Xho I restriction sites, respectively.

ISSN: 1743-422X