NFкB and CREB were recruited to the Egr-1 promoter upon infection. a.) Chip assay was performed using antibodies against NFкB and CREB. Lane1: Noninfected SIRC cells. Lane 2: SIRC cells infected with HSV-1 at a MOI of 5. Lane 3: HEK293 cells infected with virus at a MOI of 5. PCR was performed using primers for the Egr-1 promoter. For comparison, input controls (lanes 1-3) indicated the PCR products from samples prior to immunoprecipitation. b.) Over-expression of CREB protein (37 kda) was seen in VERO cells infected with HSV-1 (lane 2) when compared to the mock-infected control (lane 1).