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Figure 3 | Virology Journal

Figure 3

From: Yellow fever virus envelope protein expressed in insect cells is capable of syncytium formation in lepidopteran cells and could be used for immunodetection of YFV in human sera

Figure 3

E protein detection of vSynYFE-infected insect cells by fluorescence microscopy. Tn5B cells were infected with vSynYFE (10 pfu/cell) and at 72 h p.i., cells were fixed with ice cold acetone and incubated with a polyclonal anti-FLAV antibody (conjugated with fluorescein isothiocyanate) and viewed in a fluorescence microscope. (A) Tn5B cells mock infected (bright field). (B) Tn5B cells infected with vSynYFE showing multinucleated syncytial cells (bright field). (C) Tn5B cells mock infected (fluorescence). (D) Tn5B cells infected with vSynYFE showing multinucleated syncytial cells (fluorescence). In A, B, C and D, Bars = 30 μm.

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Virology Journal

ISSN: 1743-422X