Figure 9

Expression of Fas ligand on CD4 negative T cell from mice. The CD4+ T cells from infected and control mice were purified at different hours post infection (PI). The cells were stained with anti mouse Fas ligand antibody conjugated with allo-phycocyanin (APC). The cells negative for CD4 receptors were gated and the percentage of CD4 negative T cells expressed the Fas ligand were calculated by formula mentioned in methods. The biexponential transformation of dot plot represents the percentage of CD4- population which showed significant changes in expression of Fas ligand at different hour post infection (PI). ^a p < 0.05