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Figure 5 | Virology Journal

Figure 5

From: Immune regulation in Chandipura virus infection: characterization of CD4+ T regulatory cells from infected mice

Figure 5

In vitro Chandipura antigen specific proliferation of CD4+CD25+ T cells (T regs) from infected and control mice. The double positive cells were purified and stained with CFSE and co cultured with splenic macrophages pulsed with Chandipura antigen. The proliferation was calculated at 72 h post infection by proliferation module in Flow Jo software. It is evident that proliferation in vitro because their level of the CFSE dye, which is divided equally among daughter, cells upon cell division has decreased. The percentage of CD4+CD25+T cells in different daughter population was calculated. The values are Mean ± SE of triplicate.

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Virology Journal

ISSN: 1743-422X