Figure 5From: Herpes simplex virus induces the marked up-regulation of the zinc finger transcriptional factor INSM1, which modulates the expression and localization of the immediate early protein ICP0Subcellular localization of ICP8 in INSM1-expressing cells. HEp-2 cells were transfected with plasmids encoding Myc-tagged INSM1-FL and deletion mutants (N and C). At 20-24 h after transfection, cells were infected with HSV-1(F) (MOI = 3), fixed 18 h after infection, and reacted with anti-Myc and anti-ICP8 antibodies as described in Methods.Back to article page