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Figure 3 | Virology Journal

Figure 3

From: Herpes simplex virus induces the marked up-regulation of the zinc finger transcriptional factor INSM1, which modulates the expression and localization of the immediate early protein ICP0

Figure 3

Expression of INSM1 and INSM1 deletion mutants. (A) Schematic diagram of INSM1 domain structure. INSM1 has two proline-rich domains (P, amino acid residues 43-58 and 75-84), one NLS (N, amino acids residues 221-246) and five C2H2 zinc finger motifs (Z). HEp-2 cells were transfected with FL or deletion mutant INSM1 expression vectors and incubated for 24-48 h prior to analysis by Western blotting (B) and confocal microscopy (C). The positions of mutant proteins detected by polyclonal antibodies are shown. Anti-Myc rabbit polyclonal antibody (B, right ) and anti-INSM1 rabbit polyclonal antibody (B, left ) were used to detect full-length and mutant proteins, respectively. The asterisk indicates a non-specific band.

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Virology Journal

ISSN: 1743-422X