Figure 1

INSM1 expression in HSV-infected cells. (A) NHEK cells were infected with wild-type (WT) HSV-1, WT HSV-2, and their US3 deletion mutants (multiplicity of infection, MOI = 3), and total RNA was extracted 9 h after infection. INSM1 mRNA was amplified by RT-PCR as described in Methods. GAPDH was used as a control. Data represent two independent experiments. (B) HaCaT cells were infected with HSV-1 or UV-inactivated HSV-1 (MOI = 3), and total RNA was extracted for RT-PCR at 3 and 9 h after infection. (C) HEp-2 cells were infected with WT HSV-1, WT HSV-2, and their US3 deletion mutants (MOI = 3), and total RNA was extracted for RT-PCR at 9 h after infection.