Intracellular localization of DEV pUL55 in DEV-infected cells at 5.5 h, 49 h, 54 h post-infection. J-L. DEV-infected cells were fixed at 45.5 h, 49 h, 54 h post-infection. Samples were incubated with anti-pUL55 serum and subsequently stained with fluorescein isothiocyanate(FITC)-conjugated secondary antibody. Nuclei were counterstained with DAPI(blue). The merged fluorescence microscopy image of DEF are shown in panels J-L with high magnification (600×).