Intracellular localization of DEV pUL55 in DEV-infected cells at 30 h, 35 h, 40 h post-infection. G-I. DEV-infected cells were fixed at 30 h, 35 h, 40 h post-infection. Samples were incubated with anti-pUL55 serum and subsequently stained with fluorescein isothiocyanate(FITC)-conjugated secondary antibody. Nuclei were counterstained with DAPI(blue). The merged fluorescence microscopy image of DEF are shown in panels G-I with high magnification (600×).