Figure 6

The accumulation of mutated NS1 in cytoplasmic bodies. Mutated NS1 protein accumulated in cytoplasmic bodies that did not co-localise with markers for cis -golgi (anti-GM130), ER (anti-PDI), or endosomes (anti-EEA-1), and did co-localise with a marker for lysosomes (anti-LAMP1). Vero cells infected with NS1F-ELCmut were fixed 24 h p.i. and dual labelled with markers for the indicated organelles (green) and anti-NS1/2 polyclonal antiserum (red). Overlay images were generated using Photoshop.