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Figure 4 | Virology Journal

Figure 4

From: Mutation of the elongin C binding domain of human respiratory syncytial virus non-structural protein 1 (NS1) results in degradation of NS1 and attenuation of the virus

Figure 4

The change in the intracellular distribution of mutated NS1 protein. Vero cells were infected with NS1F, NS1F-ELCmut, or mock infected and were fixed at 15 (mock fixed at 15 h alone), 24, 32 and 48 h p.i. NS1 (white arrows) was detected by dual labelling using anti-FLAG (green) and anti-NS1/2 (red) antibodies. Nuclei were stained with DAPI and overlay images generated using Photoshop. Representative of 3 separate experiments.

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Virology Journal

ISSN: 1743-422X