Antiviral effect of GNA against HCVpp in liver cells. HCVpp was produced in HEK 293 T cells and collected in media after filtration in 0.45 micron filter. (a) GNA was incubated with HCV pseudo particle at 37°C for 1 hr. After 1 hrs Huh-7 cells were infected with pseudo particle of HCV 3a genotype in the presence and absence of GNA per well and incubated for additional 48 hrs. At the end of incubation period protein were isolated and analyzed by western blotting with anti E2 monoclonal antibody and GAPDH serve as internal control. (b) GNA was incubated with HCV pseudo particle at 37°C for 1 h. After 1 h Huh-7 cells were infected with pseudo particle of HCV 3a genotype in the presence and absence of different concentrations of GNA and incubated for 3 hrs. After 24 h cells were lysed and luciferase activity was determined by using a luminometer. Luciferase activity is not reported as an absolute value, but is calculated relative to the 'no drug' condition and reported on the y-axis as a percentage. Results are represented as the average and standard error for three independent experiments. P value > 0.05 vs control was considered as statistically significant.