Identification of the recombination vector pET-32a(+)-gI. A. PCR product of the fragment of DEV gI detected by 1% agarose gel electrophoresis. Lane M, DNA marker(in bp); Lane 1, PCR product of the DEV gI. B. DEV gI gene encoding DNA sequence was cloned into pET-32a(+) prolaryotic expression vector as described in materials and methods. The construct was digested with two restriction enzymes. Lane M(in bp), DNA marker; Lane 1, BamHI and XhoΙ generating two restriction fragments; Lane 2, BamHI generating one restriction fragment.