Transient expression results of P6 deleted derivatives. The upper two panels indicate the distribution of P6 deletions fused with GFP expressed in the onion epidermal cells, showing that P6△580-620-GFP and P6△615-655-GFP have a diffuse fluorescence pattern while P6△403-440-GFP forms numerous VLS. GFP was detected with excitation at 488 nm and emission capture at 550-590 nm. Bars, 20 μm. The lower two panels indicate the distribution of DsRed2-fused P6 deletions expressed in the epidermal cells of N. benthamiana leaves. Similarly, Both DsRed-P6C△580-620 and DsRed-P6C△615-655 show a diffuse and weak red fluorescence distribution whereas DsRed-P6C△403-440 forms VLS. Red fluorescence was detected with excitation at 543 nm and emission capture at 570-600 nm. Bars, 50 μm.