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Figure 2 | Virology Journal

Figure 2

From: Rice black-streaked dwarf virus P6 self-interacts to form punctate, viroplasm-like structures in the cytoplasm and recruits viroplasm-associated protein P9-1

Figure 2

Mapping of the P6 region involved in P6 self-interaction. (A) Schematic representation of P6 and P6 truncations in the study. The full-length P6 (spanning residues 1 to 792) and P6 truncations are indicated by open bars. The P6 domain (approximately from position 400 to 675) homologous to SMC ATPase is indicated by the gray bar and the deleted regions by the dashed lines. The numbers denote P6 amino acid positions. The ability of P6 truncations to interact with intact P6 in YTH assays is indicated in the middle (+, positive; -, negative). The VLS-forming abilities of the different P6 derivatives are shown on the right (+ + +, abundant and large VLS; + +, moderate in size and number; +, few in number; -, negative with diffuse distribution; ND, not determined). (B) Homologous interaction between intact P6 and P6 deletions in YTH assays. All truncations harbouring this region were able to interact with intact P6. As their N and C termini approached this region, the interaction ability was decreasing.

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Virology Journal

ISSN: 1743-422X