Claudin-1 required for HCV virus entry has high potential for phosphorylation and O-glycosylation

Table 2 Predicted phosphorylation and O-glycosylation sites on Claudin-1 protein

Substrate	Position	Phosphorylation prediction		Kinase prediction		O-glycosylation prediction		Surface accessibility
		Netphos	Disphos	NetphosK	Kinasephos	YinOYang	OGPET	NetSurfP
Ser	24	N	N	-	-	N	HP	B
Thr	25	N	N	CDC2	-	N	LP	B
Ser	34	N	N	PKA	-	N	LP	B
Thr	42	N	N	-	MDD	N	VHP	B
Ser	53	N	N	-	-	N	LP	B
Ser	56	N	N	DNAPK	PKG	N	VHP	B
Ser	58	P	N	PKC	ATM, IKK	N	HP	E
Thr	59	N	N	-	PKG	N	VHP	E
Ser	69	N	N	PKA	-	N	LP	B
Ser	74	N	N	-	CKI	N	LP	E
Ser	75	N	N	-	CKI	N	HP	E
Thr	76	N	N	-	CDK	N	LP	B
Thr	80	N	N	-	-	N	LP	B
Thr	99	P	N	PKC	-	N	LP	B
Thr	137	N	N	-	-	N	LP	B
Thr	153	Y	N	MAPK, GSK3	CDC2, MAPK	N	VHP	B
Thr	167	N	N	PKC, CDC2	-	N	VHP	B
Ser	173	N	N	PKA	-	N	LP	B
Ser	185	N	N	CDC2	-	N	LP	B
Thr	190	P	N	PKA	PKA	N	HP	E
Thr	191	Y	Y	PKG	PKC, PKA	Y	LP	E
Ser	192	Y	P	-	PKA, IKK, PKB	Y	LP	E
Thr	195	Y	Y	MAPK, CDC5	PKC, MAPK, CDK	N	VHP	E
Ser	205	Y	Y	PKG	CDC2	Y	VHP	E
Ser	206	Y	Y	PKC	-	Y	VHP	E

Y = yes (threshold > 0.5), P = probable (threshold > 0.1~0.5), N = No (threshold < 0.1), B = Buried surface, E = Exposed surface, VHP = very high potential (threshold ≥ 1.0), HP = high potential (threshold >0.8 < 1.0), LP = low potential (threshold <0.8)

ISSN: 1743-422X