Figure 6
From: Monoclonal antibody-based serological methods for detection of Cucumber green mottle mosaic virus
Sensitivity of IC-RT-PCR for the detection of purified CGMMV (A) and CGMMV-infected plants (B). A: Purified virions with two-fold diluted from 1:5000 to 1:2560000 (from left to right). The original purified virus concentration was 1.28 μg mL-1. The lane 1 was diluted at 1:5000, corresponding to 256 pg of purified virus. The lane 9 was diluted at 1:1280000, corresponding to 0.1 pg of purified virus. B: CGMMV-infected leaf tissue saps with two-fold dilution from 1:200 to 1:819200 (w/v, g mL-1) (Lanes 1-11). Lane 12 was positive control and lane 13 was negative control. The original cucumber tissue extracts concentration was 0.05 g mL-1. M: 1 kb DNA marker.