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Table 4 Reproducibility of the duplex real-time PCR assay

From: Development of a new duplex real-time polymerase chain reaction assay for hepatitis B viral DNA detection

Reproducibility Target HBV RNA (IU/ml) Number of determinations Mean Ct SD CV (%)
Intra-assay 105 10 24.15 0.24 0.83
  104 10 27.09 0.34 1.07
  102 10 33.31 0.49 1.25
Inter-assay 105 10 24.05 0.27 1.02
  104 10 27.05 0.23 0.77
  102 10 33.23 1.02 2.73