Development of a new duplex real-time polymerase chain reaction assay for hepatitis B viral DNA detection

Table 4 Reproducibility of the duplex real-time PCR assay

Reproducibility	Target HBV RNA (IU/ml)	Number of determinations	Mean Ct	SD	CV (%)
Intra-assay	10⁵	10	24.15	0.24	0.83
	10⁴	10	27.09	0.34	1.07
	10²	10	33.31	0.49	1.25
Inter-assay	10⁵	10	24.05	0.27	1.02
	10⁴	10	27.05	0.23	0.77
	10²	10	33.23	1.02	2.73

ISSN: 1743-422X