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Figure 1 | Virology Journal

Figure 1

From: Expression of recombinant Araraquara Hantavirus nucleoprotein in insect cells and its use as an antigen for immunodetection compared to the same antigen expressed in Escherichia coli

Figure 1

Expression, purification and antigenic analysis by western-blot of recombinant Nucleoprotein, expressed in Baculovírus expression system. A. Polyacrilamide gel electrophoresis 12,5% stained with Coomassie blue showing the expression of recombinant N protein. 1. BenchMark Protein Ladder (Bio-rad-USA), 2. Crude extract from uninfected Sf9 cells, 3. Crude extract from infected SF9 cells with recombinant Baculovirus. B. Polyacrilamide gel electrophoresis 12,5% stained with Coomassie blue showing the purification of recombinant N protein. 1. BenchMark Protein Ladder (Bio-rad-USA), 2/3. Purified recombinant N protein. C. Western-blot showing the recombinant N protein detection by HCPS serum sample. 1. BenchMark Protein Ladder (Fermentas-BRL), 2. Crude extract from infected SF9 cells with recombinant baculovirus showing the detection of recombinant N protein, 3. Crude extract from uninfected SF9 cells without detection of recombinant N protein.

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Virology Journal

ISSN: 1743-422X