Detection of NS3 in Purkinje cells. One-week-old sucking mice were infected with the SH-JEV01 strain of JEV (1.0 × 106 PFU). Samples were collected from the cerebellum of mice showing the neurological signs and stained immunohistochemically with the anti-NS3 antibody. The nuclei were counter-stained by hematoxylin. Cerebellar samples from the mock-infected mice (Mock) were used as controls. Arrows indicate Purkinje cells. Bar, 50 μm.