Figure 2

DNA shuffling of gene E (A), nucleotide and amino acid sequences(B) and lysis efficiency comparisons of two high-efficiency lysis plasmids and four non-lytic plasmids (C). A1: 276 bp lysis gene E amplified from bacteriophage PhiX174. A2: Electrophoresis of the DNA fragments generated from DNase I digestion of the lysis gene E fragment. A3: Primerless PCR products. A4: Primer PCR products. B: Sequence comparison of the recombinant plasmids. Mutant sites were boxed; deletion sites were signed with strigula. C: lysis efficiency comparisons of the recombinant plasmids. pBV-E, pBV-ΔE: lysis gene E and ΔE was inserted into Eco R I- and Bam H I sites of pBV220, respectively; control: pBV220