Different linkages in the long and short regions of the genomes of duck enteritis virus Clone-03 and VAC Strains

Table 2 Sequences of oligonucleotides primers used for PCR amplification

Primer	Sequences (5'-3')	Position^a
L1	AGTCCAGTCATCTCCATCCG	1,562-1,581
L2	ACGATTTGGCTGTGCTGTAG	1,730-1,749
L3	CTGTCTTAAGGTTAGGGCTGGC	4,532-4,553
L4	GTAGGAAATATTGAGCCGAG	4,826-4,845
L5	TTCTGATGTTTTGGCAAGCC	7,578-7,597
L6	CAGAATGGCGCTTTGTTTGG	7,669-7,688
L7	TTGAAGATAGGTTGCTCGTAG	11,272-11,292
L8	ATACAGGAAAATTAACGAT	11,367-11,385
L9	ATGTAGCAGTTTGTTCAAAC	14,193-14,212
L10	AAGATGAGTCAACACCGAAGG	14,445-14,465
L11	TATTCCATCCAGTTGCTCCC	17,732-17,751
L12	CTTGTAAAGCTGGCCGCTAC	17,978-17,997
L13	CGATCTGCTTTCGCTTTCCG	21,725-21,744
L14	TAGCTGGTATGGCAACAATG	21,895-21,914
L15	GAAGTTAACGGAGGAAGTATT	26,043-26,063
L16	GCATATAATTACTACGCAACC	26,165-26,185
L17	GTCATCCTTGTTATGTTGA	29,697-29,715
L18	CCTACTTGGTGGTCGGCCA	29,795-29,813
L19	CAGGATTTGATAACTAACC	34,266-34,284
L20	ATAAGCGCACTAGATGGCAG	34,473-34,492
L21	TGAACGGACCTTTGCTAATGAC	37,793-37,813
L22	GAGGGGTGGTACTGGTTCCG	38,120-38,139
L23	CCTACAATAACCTGGGAACT	40,770-40,789
L24	GATCTTGTCCGATGGGGATG	40,949-40,968
L25	ATGGGACAGTCCCTACCGTTGGCCTCGATTCAAAGCTTCTCAG	1-43
L26	GGACTGCAGGCCTTTCAACGGCCCCTGATATGGCTACTATGTC	18,716-18,758r^b
L27	GCCAGCCCTAACCTTAAGACAG	4,532-4,553r
L28	CAACCCCGCCCACAATAAGC	4,043-4,062
L29	GCTTACATGCTTTTCCCCGC	8,712-8,731r
L30	CGAACCGTCACAGTCTGCAG	8,592-8,611
L31	GCCGCGCAGTAAGTACTCAG	13,315-13,334
L32	TGCCGATATCATTGGTTCAT	13,212-13,231
N1	TATAGGTTT(C/A)TGTT	NP^c
N2	CTTTTGGAGCTG	NP
N3	GAATGTGA(A/G)AA	NP
N4	CATGTCTGCCGA	NP

^a the position is according to the sequence of the whole fragment of 42,897 bp.
^b r indicates the reverse direction.
^c NP indicates no prediction.

ISSN: 1743-422X