Figure 1

The comparison of genomic organization between the DEV Clone-03 strain and the published VAC strain, together with the PCR strategy. The upper part shows the genomic organization of sequences corresponding to those in the present study in the published sequences of the genome of the DEV VAC strain. The genomic organization of the presented fragment in the DEV Clone-03 is listed below. The red and blank arrows indicate the ORFs, with different colours to make two adjacent ORFs evident. The pink arrows indicate the ORFs that were not detected in other heprsviruses but were detected in DEV. The dark blue indicates the UL region; the dashed gray boxes in the genome of DEV Clone-03 indicate uncertain regions. The PCR strategy used to obtain the fragment is depicted at the bottom. The blue arrows indicate the twelve overlapping fragments that were obtained to form a continuous DNA fragment; the primer positions and amplification directions are also shown. The arrows indicate the genes and the interval shows the relative position of the two adjacent ORFs. The confirmation PCR strategy is depicted in the centre. The bars indicate the PCR product with the primers embedded. The green ellipse indicates the predicted origins of replication (oriS).