ELISPOT results from AHB patients. Sequence of Pol575-58 is FLLSLGIHL, sequence of Env348-357 is GLSPTVWLSV, sequence of Core18-27 is FLPSDFFPSV, sequence of HCV Core132-140 is DLMGYIPLV; all are HLA-A0201 limited. Medium without stimulation or cells was used for negative control, PHA for positive control. 2 × 10E5 cells per well was used for the negative and peptide stimulated groups, 1 × 10E4 cells per well was used for the positive control. The final concentration of peptide was 10 μg/ml, PHA 4 μg/ml. HLA-A0201 positive patients during the first week of hospitalization were chosen as the research subjects.