Figure 4
Pathological reduction by treatment of MDV with peptides in chicken embryos. (a) Photos show pathological changes induced by treatment of virus with or without peptides from chicken embryos (at 10 × 20 magnification, arrows indicate gray lesions). These representative images show normal membrane (no treatment), 100% lesion formation (infected by 103 pfu of MDV without peptides) and 0% lesion formation (103 pfu of MDV with 1.0 mM gHH2 in co-treatment assay, i.e., 100% lesion formation inhibition), respectively. (b and c) The infectivity rates (proportion of lesion formation) in the presence of different peptides at various concentrations (0.1, 0.5, 1.0 mM) for co-treatment (Fig. 4b) and post-treatment (Fig. 4c) assays. Peptides gHH1, gHH2, gHH3, gHH5, gBH1, NHR2, gHH6, and NHR1 are shown from left to right, respectively.