Figure 3

Time-of-addition studies. HIV-1 NL4-3 virions (MOI = 0.01) were added to HeLa37 cells at time zero. A final concentration of 10 μg/mL of P. vulgaris Ames 27748 extract was added to the cultures at times noted and extract was maintained on the cells for the remainder of the infection. Cells were fixed and immunostained for viral antigens at 40 hours following initiation of the experiment. Data are represented as the percent of control wells (Con) that did not have extract added. Each experiment was performed in triplicate and independently performed three times. Shown are the mean and standard error of the mean for each time point.