Identification of new, emerging HIV-1 unique recombinant forms and drug resistant viruses circulating in Cameroon

Table 1 List of PCR primers used for amplification of gp41, p17 and pol genes of HIV.

Gene	Primer	Reaction	Sequence	HXB2 coordinates	Size in bp
gp41*	gp40F1 gp41R1	1^st PCR	TCTTAGGAGCAGCAGGAAGCACTATGGG AACGACAAAGGTGAGTATCCCTGCCTAA	7840-8300	461
	gp46F2 gp47R1	2^nd PCR	ACAATTATTGTCTGGTATAGTGCAACAGCA TTAAACCTATCAAGCCTCCTACTATCATTA
p17*	pL393 pL392	1^st PCR	AAGGGTACTAGTAGTTCCTGCTATG GCTGAAGCGCGCACGGCAAGAG	761-1437	670
	p17-1033 p17-1048	2^nd PCR	TCTATCCCATTCTGCAGCTTCCTCATTGAT TTTGACTAGCGGAGGCTAGA
pol	ANA corr SP5R	1^st PCR	CAGGAGCAGATGATACAGTATTAG ATTTATCAGGATGGAGTTCA	2390-3229	840
	AOA Corr SP5F-r	2^nd PCR	GATAGGGGGAATTGGAGG AATGGAGGTTCTTTCTGATGT

* These primer sets were shown to amplify nucleic acid from Cameroonian viruses efficiently in previous studies [13].

ISSN: 1743-422X