Figure 1
From: Gene silencing in HIV-1 latency by polycomb repressive group
Histone and HDAC profiles in HIV-1 latently infected A3.01derived cells. A) Expression levels of four core histone proteins were investigated by western blot. Thirty ug of nuclear extract were loaded and immunoblotted using antibodies specific to H2A, H2B, H3 and H4. Anti-laminB was used as a loading control. B) The acetylation levels of four core histone proteins were quantified using the H2A-, H2B-, H3- and H4 -PathScan acetylated histone sandwich ELISA kit. Error bar represent the S.D. from the mean. C) mRNA levels of HDACs in each cell line were measured with reverse transcriptase PCR (RT-PCR). GAPDH was used as a control. D) Protein levels of HDAC1, HDAC2 and HDAC3 were investigated by western blot using 30 ug of nuclear extract. Anti-laminB was used as a loading control.