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Figure 3 | Virology Journal

Figure 3

From: Treatment with a neutralizing anti-murine interleukin-17 antibody after the onset of coxsackievirus b3-induced viral myocarditis reduces myocardium inflammation

Figure 3

IL-17, IL-6 and TNF-α mRNA transcription in VMC myocardium and protein level in the serum. A. Representative images showing semiquantitative RT-PCR for IL-17, IL-6 and TNF-α transcription. 1. Normal group; 2. IL-17mAb group; 3. Isotype control group; 4. PBS group. B, Densitometric quantitation of the PCR bands showed that, among the IL-17mAb, isotype control and PBS groups, the mRNA expression did not differ with each other; C, The levels of serum IL-17 in different groups, measured by ELISA. The IL-17 protein levels were 11.81 ± 2.66, 22.56 ± 3.68, 33.63 ± 5.50, 33.68 ± 6.13 pg/ml in normal, IL-17mAb, Isotype control and PBS group respectively. D, The levels of serum IL-6 and TNF-α in different group, measured by ELISA. The IL-6 levels were 23.15 ± 8.59, 71.28.50 ± 15.80, 77.81 ± 6.54, 78.18 ± 6.26 pg/ml, and the TNF-α levels were 27.80 ± 4.52, 64.24 ± 6.71, 67.89 ± 5.25, 63.45 ± 2.71 pg/ml respectively. Normal group (n = 8), IL17mAb (n = 7), isotype contro(n = 4), and PBS group(n = 5).*P < 0.05, △P > 0.05. Data are mean ± SD.

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Virology Journal

ISSN: 1743-422X