Figure 9
From: Establishment of persistent infection with foot-and-mouth disease virus in BHK-21 cells
Infectivity and virulence assays of FMDV-Ops and wt. (A) Plaque assays. Plaque assays were designed to compare the infectivity of FMDV-Ops with wild type. Confluent BHK-21 cells in 6-well plates were infected with FMDV wt, FMDV-Op8 and FMDV-Op48 at equal amounts of viral RNAs and were overlaid with 3 ml maintenance medium (2% FBS) containing 0.8% purified agarose and incubated for 3d. Plaques were stained with 1 ml 1% crystal violet in 10% paraformaldehyde. A. FMDV wt. B. FMDV-Op8. C. FMDV-Op48. (B) TCID50 assays. TCID50 assays were designed to compare the virulence of FMDV-Ops with wild type. The TCID50 value of FMDV-Ops and wt were calculated every 12 h from 36 h.p.i. till 72 h.p.i.