Figure 7
From: Establishment of persistent infection with foot-and-mouth disease virus in BHK-21 cells
Cell viability of BHK-21 cells and BHK-Op cells in the presence or absence of 20 mM ammonium chloride. MTT assays were used to determine the viability of BHK-21 cells and persistent BHK-Op cells treated with 0 mM (negative control for this assay) or 20 mM ammonium chloride. Cell survival rate was measured at three different time points: 24 h, 48 h maintenance in ammonium chloride and 48 h maintenance in ammonium chloride then in fresh 10% FBS MEM for 24 h. The results are detected from two independent experiments.