Effect of HSV-2 infection on HIV-1 entry into MDMs. Single round replication HIV-1 recombinant particles pseudotyped by different envelopes (HIV-env) were employed to transduce uninfected (HSV-2 -) or infected (HSV-2 +) MDMs, 72 hours post HSV-2 infection. The entry into cells was analyzed by CAT assay performed on the cell lysates, normalized for protein content. The percentage of CAT conversion is reported and represents the mean of four independent experiments. The error bars represent the standard deviation. Statistically significant differences between HSV-2 infected and un-infected cells, identified by employing the unpaired t-Student test, are indicated with the star (p < 0.05).