Table 2 Oligonucleotide primers used in RT-PCR amplification and nucleotide sequencing of 07V063

5'endFW

atgatgtgtagggtattccccc

1-22

Orf1univFW

ccctttaaccatgtctggc

111-130

Orf1-1fw

catcc gggtg ctgctgg ctt

336-355

Orf1-2fw

ggag ccaccc acgtgtt gac

681-701

Lav49fw

aatcaatggtattcgtgctg

1072-1091

Orf1-3-fw

tcaat gcctacaa ctgcccg

1631-1650

Orf1-4-fw

cttgta taaa ttgct attgg

1988-2007

Orf1-5-fw

acaa cagg cctc gtaa ggg

2472-2490

Lav73fw

aaaacttggcgctgcacgtc

3102-3121

Orf1-6fw

ggtcc atta gcca gcgcct

3451-3469

Orf1-7fw

cttgag cagcg ccaa cattg

3686-3705

Lav33fw

ggtgttggcacggcgagag

4129-4147

Orf1-8fw

catgg ctgtt gccca agtgt

4538-4557

Orf1-9fw

ttgt gctt acgcc tggccca

4859-4878

Orf1-10fw

ggcgac tcct ataat cgtat

5364-5383

Orf1-11fw

ccaa gcac ttcg cagg tccg

5701-5720

Orf1-12fw

ggctt ggctg ccgaaa tcgg

6096-6115

Orf1-13fw

aatgaa gggag tctt gtcta

6566-6586

Lav92fw

gtgtatccctcggctaccac

6891-6911

Orf1-14fw

catta gtcaa cttcaa ggtt

7280-7299

Orf1-15fw

gga ccc tga gcgg catgaa

7765-7783

Lav12fw

ccaagaactccatggcaggt

8172-8191

Orf1-16fw

ggaaaaacaaattcaaggag

8442-8461

Orf1-17fw

tccag cccatg ctggt ata

8817-8835

Lav51fw

gtgtttgtttcactcacact

9316-9335

Amp6fwint

catcagaccatgtttgacat

9764-9783

Orf1-18fw

aaggc caggaa cacca gggt

10136-10155

Orf1-19fw

cccagta tttgca ccttt gc

10633-10652

Orf1-20fw

cggccgta cttgc aaccag

11132-11150

Orf2afw

gts aca cck tat gatta cg

11387-11406

LavORF2aseqfw

gtgttcgacaacgcccacacgc

11577-11598

Orf3fw

agcc taca gta caa ca ccac

12234-12253

LavORF3seq1fw

agcgttgagctcatcttccc

12261-12280

Orf4fw

cgg ccc ait tcc atccigag

12672-12691

Orf5Pesfw

tga tca cat tcg gtt gct

13320-13337

Orf6fw

tacc aa ctt tc ttc tggac

13838-13856

Orf7fw

tgg cccc tgccc aic acg

14328-14345

Orf1-1-rev

gtcaa cacgt gggtgg ctcc

701-681

Lavgsprev

cgacttgacattctagtcca

900-881

Orf1-2-rev

agat gcca aacgg acgaa cc

1304-1285

Orf1-3-rev

gcag cctt cgga gcag acgc

1796-1777

ORF1-4-rev

cggtg aaca cgag acacc tg

2252-2233

Orf1-5-rev

gctg atgt tgtc ggatt ctg

2615-2596

Orf1-6-rev

ctggg aaca ggagg cgg tgt

3202-3182

Orf1-7-rev

gggttgg atg gagtc gagaa

3730-3711

Lav33rev

ccccaacacttgtgacaacg

3982-3963

Orf1-8rev

gt ccgag tccac tacaatc

4403-4385

Orf1-9rev

agag ttgt gccac tgct gaaa

4755-4735

Amp3intrev2

cagagaaggccggttattcct

5023-5003

Amp3intrev

gattccaatgagatcacca

5609-5591

Orf1-10rev

gctc ggac taaaa cagc tgg

5959-5940

Lav92rev

caccaatgatgatgataggg

6222-6203

Orf1-11rev

cttg caca gaca cagtttt

6720-6702

Orf1-12rev

ttcaa ggca gttg tca ggct

7190-7171

Orf1-13rev

tca ttaa gacg acacc ggaa

7406-7386

Orf1-14rev

cttg ccat cgga cacaa gg

7903-7885

Orf1-15rev

tga cacc actg agcg ccga

8396-8378

Orf1-16rev

agaca cact ggtg acggggt

8696-8676

Lav51rev

aagaaagctgggtttgtcag

8971-8952

Orf1-17rev

cggaa tctg tttcaa cacag

9460-9441

Orf1-18rev

ccagg tggtt gcaa tatcca

9944-9925

Orf1-19rev

aaaactccc gaag ttggtcg

10385-10366

Orf1-20rev

aggc ttgc tgtag tgggcat

10762-10743

Lav82rev

ttcaagctggaagtaggc

11244-11225

Orf1-21rev

tgatttt gctcc acag tgac

11741-11722

Orf2arev

tcatr ccc tatt y tgc acca

12558-12539

Orf3rev

agaa aa gg cacgc ag aaa gca

13184-13165

Orf4rev

cattcagctcgcataicgtcaag

13569-13547

Orf5Pesrev

ggg cgt ata tca tta tag gtg

14100-14079

Orf6rev

acccagc aa ctgg cacag

14606-14589

Orf7rev

tcg ccc taa ttg aa tagg tga

14966-14946