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Figure 1 | Virology Journal

Figure 1

From: Hepatitis E virus ORF2 protein over-expressed by baculovirus in hepatoma cells, efficiently encapsidates and transmits the viral RNA to naïve cells

Figure 1

In vitro transcription of HEV genomic replicon. The Bgl II-linearized plasmid, pSK-GFP(replicon), (5 μg) was transcribed in a 50 μl reaction volume and cooled on ice. A 2.5 μl of the RNA mixture was checked for RNA integrity and semi-quantitation on a 1% agarose gel, containing ethidium bromide. DNA. mr.(standard DNA marker); Neg.cont.(negative control).

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Virology Journal

ISSN: 1743-422X