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Figure 2 | Virology Journal

Figure 2

From: Positive selection in the hemagglutinin-neuraminidase gene of Newcastle disease virus and its effect on vaccine efficacy

Figure 2

Identification of HN protein expressed in the recombinant fowlpox virus (rFPV) by indirect immunofluoresence assay (IFA). Chick embryo fibroblast (CEF) cells were infected with rFPV-LaSHN (A), rFPV-JS6HN (B), or wild-type parental FPV (C) at a multiplicity of infection (MOI) of 0.01. Sixty hours post-infection, IFA was performed using polyclonal anti-NDV chicken serum and FITC-conjugated rabbit anti-chicken IgG as primary and secondary antibody, respectively. The fluorescence was observed under an inverted fluorescence microscope.

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Virology Journal

ISSN: 1743-422X