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Figure 3 | Virology Journal

Figure 3

From: The duck hepatitis virus 5'-UTR possesses HCV-like IRES activity that is independent of eIF4F complex and modulated by downstream coding sequences

Figure 3

Translation initiation of putative DHV-1 IRES is not inhibited by 4E-BP1. The dicistronic capped RNAs indicated in the figure were added to Flexi RRL in the presence of increasing amounts of purified recombinant 4E-BP1 protein (0, 9, 18 and 36 μg/ml) and the in vitro translation of the reporter proteins was evaluated measuring 35S-methionine incorporation. (B) Western-blot analysis of the recombinant 4E-BP1 protein added in the different conditions.

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Virology Journal

ISSN: 1743-422X