Phenotypic and genotypic variations within a single bacteriophage species

Table 1 Overview of ϕKMV-related bacteriophages included in this study, all infecting P. aeruginos a.

Phage	Isolated in	Genome (bp)	# ORFs	DTR length	GC (%)	ϕKMV identity^a	Accession number	K_adsorption(ml/min)^b	Latent period (min)	Burst size (PFU/cell)	K_APS/PT5^c	K_APS/PNC101^c
ϕKMV	Moscow, Russia	42,519	52	414	62.3	100	NC_005045	1.6 × 10^-8	28	116	259	849
LKD16	Leuven, Belgium	43,200	54	428	62.6	83	NC_009935	-	27	120	87.5	-
LUZ19	Leuven, Belgium	43,548	54	472	62.2	89	NC_010326	1.2 × 10^-7	22	122	-	-
ϕKF77	Moscow, Russia	43,152	53	454	62.8	90	NC_012418	1.5 × 10^-8	23	129	56.2	-
PNM	Tbilisi, Georgia	42,721	51	422	62.3	94	-	ND	ND	ND	-	23
PT2	Tbilisi, Georgia	42,961	54	488	62.0	97	NC_011107	9.2 × 10^-9	21	116	737	51
PT5	Tbilisi, Georgia	42,954	52	413	62.0	95	NC_011105	7.3 × 10^-9	21	134	598	679
PT6	Tbilisi, Georgia	ND	ND	ND	ND	ND	-	2.1 × 10^-8	25	166	257	857
PNC101	Tbilisi, Georgia	ND	ND	ND	ND	ND	-	ND	ND	ND	60	753
LUZ2	Leuven, Belgium	ND	ND	ND	ND	ND	-	ND	ND	ND	270	-
LKA1	Leuven, Belgium	41,593	56	298	60.9	ND	NC_009936	3.9 × 10^-9	41	225	-	-

^a Identity with ϕKMV, expressed as % identical nucleotides according to the Stretcher algorithm (EMBOSS suite).
^b The adsorption constant k was calculated from the unabsorbed phage at a multiplicity of infection of 1:1 by applying the formula k_a = 2.3/Bt log P₀/P, where P₀ is initial number of phage, P is number of unabsorbed phage particles after time t and B is number of bacteria (4). ND, not determined.
^c Serological neutralization assay using PT5 and PNC101 APS. Dilutions (1/50- 1/1000) of APS were mixed with 10⁶ bacteriophage, samples were titered after different incubation times (5, 15 and 30 min) at 37°C. K, rate constant of neutralization, defined as k = 2.3 D/t logP₀/P, where P₀ - count of bacteriophage plaques at zero time, P - count of bacteriophage plaques after t time, D - dilution of APS.

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