Figure 3
From: Japanese encephalitis virus co-opts the ER-stress response protein GRP78 for viral infectivity
GRP78 is released into the media upon JEV infection and partially co-fractionates with the JE virion. A) Verification of GRP78 in the secretome from JEV-induced BHK-21 cells. Cell lysates or secretion medium were collected 3 days post-infection followed by SDS-PAGE for protein separation. The GRP78 was detected by anti-GRP78 specific antibody. Two independent replicates are shown for the mock- and JEV-infected conditions. B) Sucrose density gradient fraction of JE virion and GPR78. A volume of 40 μL of sample from each fraction was analyzed on SDS-PAGE followed by the detection of anti-JEV E protein and anti-GRP78 by Western blotting.