Biological and immunological characterization of recombinant Yellow Fever 17D Viruses expressing a Trypanosoma cruzi Amastigote Surface Protein-2 CD8+T cell epitope at two distinct regions of the genome

Table 1 Swiss mouse neurovirulence of YF 17D viruses

Inoculation	Dose (log ₁₀ PFU)	Mouse challenge % mortality (dead/tested)	AST ± SD (days) ^b
Control ^a	-	0 (0/30)	-
YF 17DD (vaccine)	3.0	97 (29/30)	10 ± 1.8
YF17D/NS2B3/Tc	3.0	27 (8/30)*	12 ± 4.1
YF17D/E200/Tc	3.0	3 (1/30)*	13 ± 0.0

^a The mouse control group was inoculated with only Earle's 199 medium by the intracerebral route
^b AST, Average Survival Time ± Standard Deviation (SD)
* P < 0.05, logrank test (Mantel-Cox) test

ISSN: 1743-422X