Schematic diagram of YF 17D virus genome organization and the corresponding insertion sites. The YF virus genome (A) is translated in a polyprotein of 3,411 amino acids (B), which is proteolytically processed by the viral protease complex NS2B-NS3. Open arrows indicate cleavage sites, releasing functional viral proteins in the cytosol of the cell. Dark arrows indicate host signalase cleavage sites. Open triangle indicates furin cleavage site and dark triangle indicates unknown host protease cleavage site. TEWETGQI epitope was inserted at two different positions (C): between f and g strands of the YF 17D virus E protein Domain II located at 482 amino acid of (left panel) or between duplicated flanking viral protease cleavage sites (GARR/S Glycine-Alanine-Arginine-Arginine-Serine) at the junction between NS2B and NS3 proteins (open arrows; right panel) located at 1486 amino acid of the YF 17D virus polyprotein.