Figure 1

Effects of RNAPII transcription inhibitors on influenza viral polymerase activity. 293T cells were transfected with A/WSN/33 PB2, PB1, PA and NP protein expression plasmids and a pPolI Luc-RT RNA expression plasmid, as described previously [10]. The transfected cells were then washed and replenished with media containing various concentrations of ActD (A), DRB (B), H7 (C) or EtBr (D) at six hours post-transfection. The luciferase activity of the drug-treated cells was measured with a luminometer (Victor3, PerkinElmer) using a Steady-Glo luciferase reagent (Promega) at 22 hours post-transfection. The luciferase activity of the mock-treated cells was taken as 100% polymerase activity. Data ± SE were obtained from the triplicate experiments.