Table 4 Nine pairs of primers used to generate overlapping PCR fragments from genomes of F48-like NDV strains
Fragment designation | Â | Primer sequence (5'-3') | Position | Expected product size (bp) |
|---|---|---|---|---|
A | Fa | ACCAAACAGAGAATCCGTGAG | 1-21 | 2280 |
| Â | Rb | TGGACGATTTATTGCTAAGCTTG | 2258-2280 | Â |
B | F | CAAGACTGGAGCAAGCAACT | 2219-2238 | 2003 |
| Â | R | GGAGAGGCATTTGCTATAGG | 4202-4221 | Â |
C | F | GGGCTCAGTGATGTGCTCG | 4100-4118 | 1964 |
| Â | R | ATATAGGTAATGAGAGCAGATGTG | 6040-6063 | Â |
D | F | AAATAATATGCGTGCCACCT | 5434-5453 | 1661 |
| Â | R | GAACGCAGAGTAGAAAAGAATA | 7073-7094 | Â |
E | F | CAAGAACACCTGAATTTTATCCCG | 6686-6909 | 2260 |
| Â | R | TTAGATGCCTTTGGACCTGTTTTA | 8922-8945 | Â |
F | F | TGGTTTCACTCAAAATGGTCC | 8876-8896 | 1223 |
| Â | R | ATCCCTTCTGCCATTACCTG | 10079-10098 | Â |
G | F | ACCCTTGAGTACCTAAGAGATGA | 9965-9987 | 2116 |
| Â | R | TGTCCCCATAAGCCCAGAT | 12062-12080 | Â |
H | F | AATCCTGATACCATAGAACTTGT | 11801-11823 | 2032 |
| Â | R | GTCCGAAATGTCGCTGTG | 13815-13832 | Â |
I | F | CTAGGAAGAGCCTTAATTTGAT | 13350-13371 | 1838 |
| Â | R | ACAAAGATTTGGTGAATGACA | 15167-15187 | Â |